PSIM: pattern-based read simulator for RNA-seq analysis

PSIM: pattern-based read simulator for RNA-seq analysis



Next-generation sequencing technologies (NGS) require mapping tools that are fundamental for their application. These are evaluated by the level of accuracy to be matched and read at the original location. Evaluation increases the need for a simulator to generate reads with their locations and errors, as with indel. In this paper, we propose a simulator, PSIM, that generating a set of artificial RNA segments(reads) with the expression level and errors based on a pattern-based SAM file. PSIM adopts the contour line transpose and interval section shuffle methods to generate a similar expression level. In addition, we show the similarity between a profile contour of synthesized data and a reference sequence.
[Journal of Multimedia Tools and Applications PDF] PSIM Web service